General description

Recombinant, Xanthomonas manihotis β1,3-Galactosidase expressed in E. coli. Cleaves terminal β1,3-linked galactose residues on oligosaccharides. Has >100-fold kinetic preference for β1,3-over β1,6-linkages and 500-fold kinetic preference for β1,3-over β1,4-linkages.

Recombinant, Xanthomonas manihotis β1,3-Galactosidase expressed in E. coli. Cleaves terminal β1,3-linked galactose residues on oligosaccharides. Has >100-fold kinetic preference for β1,3- over β1,6-linkages and 500-fold kinetic preference for β1,3- over β1,4-linkages.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Taron, C., et al. 1995. Glycobiology 5, 603.Wong-Madden, S.T., and Landry, D. 1995. Glycobiology 5, 19.

Packaging

500 u in Glass bottle

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 100 µM EDTA, pH 7.5.

Unit Definition

One unit is defined as the amount of enzyme that will cleave >95% of the β-D-galactose from 1 nmol Galβ1,3GlcNAcβ1,3Galβ1,4Glc-7-amino-4-methylcoumarin in 1 h at 37°C, pH 4.5 in a total volume of 10 µl.

Warning

Toxicity: Standard Handling (A)